Reporter
Part:BBa_K2082113:Design
Designed by: Carsten Hain Group: iGEM16_Bielefeld-CeBiTec (2016-10-14)
Stop-GFP (K3*) under control of constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Design Notes
Stop position was determined by FoldX modelling. Reversion of the stop codon to every other amino acid should result in a functional protein.
Source
cloning by side-directed mutagensis